Belfast, Northern Ireland, 19 September 2011 – 倒置顯微鏡添加共軛焦系統。
這個設計最關鍵的部分是包含了一個通用的部件可以確保相機、共軛焦系統的穩定性。它還賦予DSD獨特的濾片轉換功能，無需拆卸即可完成，這以前只能在倒置顯微鏡上實現。用戶不需要再組裝共軛焦系統，這樣就節省了寶貴的成像時間。
另外，這種不需要激光的技術能提供四根波長，而不是三根。由於電動化Andor AMH金屬鹵素燈光源的改進，波長轉換更加快了，達到樣品的光強更加精細，而且每個波長可獨立控制。這極大地減少了固定、活細胞成像的漂白和光毒性。
近來發表的論文證實Revolution DSD非常強大，它提供自由可調的激發、發射濾片，可以用於開發新的螢光探針，和點掃描成像相比更快速的成像速度，還可以根據您的需要選擇共軛焦或是寬視野成像。
原文出處：
http://www.andor.com/microscopy_systems/white_light_confocal/revolution_dsd/
Revolution® DSD
Laser-free confocal microscopy
Andor’s Revolution DSD is an innovative hybrid of spinning disk technology and structured illumination. This unique laser-free design delivers a budget friendly confocal solution to your laboratory. A simple device, which can even be added to an existing fluorescence microscope in your lab, the Revolution DSD means that confocal imaging can be a routine technique in your own lab. Whilst laser-free, the Revolution DSD can still achieve the optical sectioning and image quality you expect of a complex laser scanning confocal system, but with low maintenance costs. Furthermore, it does not need an expert to run it!
The DSD delivers high contrast, low background images with objective magnifications from 10x to 100x, using air, water or oil immersion lenses, making it suitable for a wide range of live cell and developmental applications.
Core components of the Revolution® DSD imaging unit include our best in class interline CCD camera,
Clara, and our new motorized DC-stabilized metal halide source. Additional optional hardware devices are available according to your research requirements. These components are integrated seamlessly to perform 5D and 6D imaging and the system can be fitted to most makes and models of inverted and upright microscopes.
| Features | Benefits |
|---|
| Real-time control and viewing | Switch wavelength and mode in real time between wide field and confocal with one mouse click. |
| Full spectrum, laser-free | Excitation 380-700 nm using Andor’s 200 W, DC-stabilized, high intensity, metal halide source. |
| Excellent confocality | Active background rejection ensures strong, clear optical sections even at low magnification. |
| High throughput | Efficient disk design delivers 50% transmission in high sectioning mode (HS) and 25% in high contrast (HC). |
| Highly cost effective | Revolution® DSD is designed for high levels of performance with easy alignment and low maintenance. |
| Ideal for live cells and embryos | Operating from 10X to 100X, makes DSD suitable for live cell, tissue and whole embryo applications. |
| Immuno, GFPs, ratio labels | Broad spectrum source and filter selection allows use of visible and NIR optical probes. |
| Integrates with any microscope | DSD connects to any side port and we offer customized supports for upright designs. Flexibility is a key goal. |
| Andor Clara – best in class camera | Clara delivers the best interline CCD camera performance with lowest read noise and extended spectral range. |
| High performance light source | 200 W DC stabilized, delivers twice the output of 120W models with 2000 hour, alignment-free lamp cartridge. |
| Active background rejection | Patented optical design combines in-focus and out-of-focus light to reject background and deliver high contrast. |
